000			02340nam a22002897a 4500
003			ZW-GwMSU
005			20250319081253.0
008			250319b |||||||| |||| 00| 0 eng d
022			_a0176-1617
040			_aMSU _bEnglish _cMSU _erda
050	0	0	_aQK711..2 JOU
100	1		_aHamo, Maya Lugassi-Ben _eauthor
245	1	0	_aCharacterization of expressed sequence tags from Lilium longiflorum in vernalized and non-vernalized bulbs _ccreated by Maya Lugassi-Ben Hamo, Carlos Villacorta Martin and Michele Zaccai
264	1		_aAmsterdam: _bElsevier GmbH, _c2015.
336			_2rdacontent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_2rdacarrier _avolume _bnc
440			_aJournal of Plant Physiology _vVolume 173
520	3		_aIn Lilium longiflorum, vernalization is both an obligatory requirement and the major factor affecting flowering time, however, little is known about the molecular regulation of this mechanism in Lilium and other flowering bulbs. Exposure of L. longiflorum bulbs to 9 weeks at 4°C greatly promoted stem elongation within the bulb, floral transition and flowering. Subtraction libraries of vernalized (V) and non-vernalized (NV) bulb meristems were constructed. 671 and 479 genes were sequenced, from which 72 and 82 proteins were inferred for the NV-V and the V-NV libraries, respectively. Much lower transcription levels and putative gene functions were recorded in the NV-V libraries compared the V-NV libraries. However, a large number of genes annotated to transposable elements (TEs), represented more than 20% of the sequenced cDNA were expressed in the NV-V libraries, as opposed to less than 2% in the V-NV libraries. The expression profile of several genes potentially involved in the vernalization pathway was assessed. Expression of LlSOC1, the lily homologue of SUPPRESSOR OF OVER-EXPRESSION OF CO1 (SOC1), an important flowering gene in several plant species, found in the V-NV library, was highly up-regulated during bulb meristem cold exposure. The subtraction libraries provided a fast tool for relevant gene isolation.
650			_aESTs
650			_aFlowering
650			_aLilium longiflorum
700	1		_aMartin, Carlos Villacorta _eco-author
700	1		_aZaccai, Michele _eco-author
856			_uhttps://doi.org/10.1016/j.jplph.2014.09.015
942			_2lcc _cJA
999			_c169365 _d169365