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| 003 | ZW-GwMSU | ||
| 005 | 20250319080857.0 | ||
| 008 | 250319b |||||||| |||| 00| 0 eng d | ||
| 022 | _a0176-1617 | ||
| 040 |
_aMSU _bEnglish _cMSU _erda |
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| 050 | 0 | 0 | _aQK711..2 JOU |
| 100 | 1 |
_aPérez, Marta _eauthor |
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| 245 | 1 | 0 |
_aEpigenetic and hormonal profile during maturation of Quercus Suber L. somatic embryos Marta Pérez, Marcos Viejo, Maite LaCuesta, Peter Toorop, María Jesús Cañal _ccreated by Marta Pérez, Marcos Viejo, Maite LaCuesta, Peter Toorop, María Jesús Cañal |
| 264 | 1 |
_aAmsterdam: _bElsevier GmbH, _c2015. |
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| 336 |
_2rdacontent _atext _btxt |
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| 337 |
_2rdamedia _aunmediated _bn |
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| 338 |
_2rdacarrier _avolume _bnc |
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| 440 |
_aJournal of Plant Physiology _vVolume 173 |
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| 520 | 3 | _aSomatic embryogenesis is a powerful alternative to conventional mass propagation of Quercus suber L. However, poor quality and incomplete maturation of somatic embryos restrict any application. Given that epigenetic and hormonal control govern many developmental stages, including maturation of zygotic embryos, global DNA methylation and abscisic acid (ABA) were analyzed during development and maturation of cork oak somatic embryos. Our results indicated that development of somatic embryos concurred with a decrease in 5-mdC. In contrast, endogenous ABA content showed a transient increase with a peak in immature E2 embryos denoting the onset of the maturation phase. A cold stratification phase was necessary for embryos to acquire germination ability, which coincided with a significant decrease in 5-mdC and ABA content. Immunohistochemical analyses showed that there was a specific spatial-temporal regulation during embryogenesis, particularly after the cold treatment. The acquisition of germination capacity concurred with a general low 5-mdC signal in the root meristem, while retention of the 5-mdC signal was mainly located in the shoot meristem and provascular tissues. Conversely, ABA immunolocalization was mainly located in the root and shoot apical meristems. Furthermore, a strong decrease in the ABA signal was observed in the root cap after the stratification treatment suggesting a role for the root cap during development of somatic embryos. These results suggest that, in addition to ABA, epigenetic control appears to play an important role for the correct maturation and subsequent germination of cork oak somatic embryos. | |
| 650 | _aABA | ||
| 650 | _aDNA methylation | ||
| 650 | _aImmunolocalization | ||
| 700 | 1 |
_aViejo, Marcos _eco-author |
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| 700 | 1 |
_aLaCuesta, Maite _eco-author |
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| 700 | 1 |
_aToorop,Peter _eco-author |
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| 700 | 1 |
_aCañal, María Jesús _eco-author |
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| 856 | _uhttps://doi.org/10.1016/j.jplph.2014.07.028 | ||
| 942 |
_2lcc _cJA |
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| 999 |
_c169363 _d169363 |
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