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022 _a0176-1617
040 _aMSU
_bEnglish
_cMSU
_erda
050 0 0 _aQK711.2 JOU
100 1 _aWang, Chii-Jeng
_eauthor
245 1 0 _aMolecular characterization of fruit-specific class III peroxidase genes in tomato (Solanum lycopersicum)
_ccreated by Chii-Jeng Wang, Yuan-Li Chan, Chin Hui Shien, Kai-Wun Yeh
264 1 _aAmsterdam:
_bElsevier GmbH,
_c2015.
336 _2rdacontent
_atext
_btxt
337 _2rdamedia
_aunmediated
_bn
338 _2rdacarrier
_avolume
_bnc
440 _aJournal of Plant Physiology
_vVolume 177
520 3 _aIn this study, expression of four peroxidase genes, LePrx09, LePrx17, LePrx35 and LePrxA, was identified in immature tomato fruits, and the function in the regulation of fruit growth was characterized. Analysis of amino acid sequences revealed that these genes code for class III peroxidases, containing B, D and F conserved domains, which bind heme groups, and a buried salt bridge motif. LePrx35 and LePrxA were identified as novel peroxidase genes in Solanum lycopersicum (L.). The temporal expression patterns at various fruit growth stages revealed that LePrx35 and LePrxA were expressed only in immature green (IMG) fruits, whereas LePrx17 and LePrx09 were expressed in both immature and mature green fruits. Tissue-specific expression profiles indicated that only LePrx09 was expressed in the mesocarp but not the inner tissue of immature fruits. The effects of hormone treatments and stresses on the four genes were examined; only the expression levels of LePrx17 and LePrx09 were altered. Transcription of LePrx17 was up-regulated by jasmonic acid (JA) and pathogen infection and expression of LePrx09 was induced by ethephon, salicylic acid (SA) and JA, in particular, as well as wounding, pathogen infection and H2O2 stress. Tomato plants over-expressing LePrx09 displayed enhanced resistance to H2O2 stress, suggesting that LePrx09 may participate in the H2O2 signaling pathway to regulate fruit growth and disease resistance in tomato fruits.
650 _aClass III peroxidases
650 _aH(2)O(2) stress
650 _aPlant hormone
700 _aChan, Yuan-Li
_eco-author
700 _aShien, Chin Hui
_eco-author
700 _aYeh, Kai-Wun
_eco-author
856 _uhttps://doi.org/10.1016/j.jplph.2015.01.011
942 _2lcc
_cJA
999 _c169290
_d169290