| 000 | 02587nam a22003017a 4500 | ||
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| 003 | ZW-GwMSU | ||
| 005 | 20250312140240.0 | ||
| 008 | 250312b |||||||| |||| 00| 0 eng d | ||
| 022 | _a0176-1617 | ||
| 040 |
_aMSU _bEnglish _cMSU _erda |
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| 050 | 0 | 0 | _aQK711.2 JOU |
| 100 | 1 |
_aPalaniyandi, Sasikumar Arunachalam _eauthor |
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| 245 | 1 | 0 |
_aMolecular cloning and characterization of the ABA-specific glucosyltransferase gene from bean (Phaseolus vulgaris L.) _ccreated by Sasikumar Arunachalam Palaniyandi, Gyuhwa Chung, Sang Hyon Kim and Seung Hwan Yang |
| 264 | 1 |
_aAmsterdam: _bElsevier GmbH, _c2015. |
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| 336 |
_2rdacontent _atext _btxt |
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| 337 |
_2rdamedia _aunmediated _bn |
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| 338 |
_2rdacarrier _avolume _bnc |
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| 440 |
_aJournal of plant physiology _vVolume 180 |
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| 520 | 3 | _aLevels of the plant hormone abscisic acid (ABA) are maintained in homeostasis by a balance of its biosynthesis, catabolism and conjugation. The detailed molecular and signaling events leading to strict homeostasis are not completely understood in crop plants. In this study, we obtained cDNA of an ABA-inducible, ABA-specific UDP-glucosyltransferase (ABAGT) from the bean plant (Phaseolus vulgaris L.) involved in conjugation of a glucose residue to ABA to form inactive ABA-glucose ester (ABA-GE) to examine its role during development and abiotic stress in bean. The bacterially expressed PvABAGTase enzyme showed ABA-specific glucosylation activity in vitro. A higher level of the PvABAGT transcript was observed in mature leaves, mature flowers, roots, seed coats and embryos as well as upon rehydration following a period of dehydration. Overexpression of 35S::PvABAGT in Arabidopsis showed reduced sensitivity to ABA compared with WT. The transgenic plants showed a high level of ABA-GE without significant decrease in the level of ABA compared with the wild type (WT) during dehydration stress. Upon rehydration, the levels of ABA and phaseic acid (PA) decreased in the WT and the PvABAGT-overexpressing lines with high levels of ABA-GE only in the transgenic plants. Our findings suggest that the PvABAGT gene could play a role in ABA homeostasis during development and stress responses in bean and its overexpression in Arabidopsis did not alter ABA homeostasis during dehydration stress. | |
| 650 | _aABA homeostasis | ||
| 650 | _aABA-glucose ester | ||
| 650 | _aAbscisic acid | ||
| 700 | 1 |
_aChung, Gyuhwa _eco-author |
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| 700 | 1 |
_aKim, Sang Hyon _eco-author |
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| 700 | 1 |
_aYang, Seung Hwan _eco-author |
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| 856 | _uhttps://doi.org/10.1016/j.jplph.2015.01.015 | ||
| 942 |
_2lcc _cJA |
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| 999 |
_c169270 _d169270 |
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