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022 _a0176-1617
040 _aMSU
_bEnglish
_cMSU
_erda
050 0 0 _aQK711.2 JOU
100 1 _aPalapol, Yossapol
_eauthor
245 1 0 _aExpression of expansin genes in the pulp and the dehiscence zone of ripening durian (Durio zibethinus) fruit
_ccreated by Yossapol Palapol, Sutin Kunyamee, Monthatip Thongkhum, Saichol Ketsa, Ian B Ferguson and Wouter G. van Doorn
264 1 _aAmsterdam:
_bElsevier GmbH,
_c2015.
336 _2rdacontent
_atext
_btxt
337 _2rdamedia
_aunmediated
_bn
338 _2rdacarrier
_avolume
_bnc
440 _aJournal of plant physiology
_vVolume 182
520 3 _aDurian (Durio zibethinus) fruit was harvested at the commercially mature stage and stored at 25 °C. Durian fruit have 3–5 longitudinal dehiscence zones (DZs) in the peel, which are up to 40 cm long and 2 cm thick in large fruit. Dehiscence started a week after harvest, was hastened by exogenous ethylene, and delayed by 1-methylcyclopropene (1-MCP), showing that it is regulated by endogenous ethylene. Three genes encoding α-expansins (DzEXP1-3) were isolated. In the expression of these genes increased, prior to dehiscence. Pulp firmness decreased during storage. The decrease was hastened by ethylene and delayed by 1-methylcyclopropene (1-MCP). Exogenous ethylene promoted gene expression of DzEXP1 both in the DZs and in the pulp. It had a smaller effect on DzEXP2 in the zones and pulp, but did not affect DzEXP3 expression. 1-MCP inhibited the expression of DzEXP1 and, somewhat less, of DzEXP2, but did not affect DzEXP3 expression, both in DZs and pulp. It is concluded that the close relationship between expression of DzEXP1 and DzEXP2 and both dehiscence and fruit softening suggests that these genes are involved in both processes.
650 _a-Methylcyclopropene
650 _aEthylene
650 _aExpansin
700 1 _aKunyamee, Sutin
_eco-author
700 1 _aThongkhum, Monthatip
_eco-author
700 1 _aKetsa, Saichol
_eco-author
700 1 _aFerguson, Ian B
_eco-author
700 1 _avan Doorn, Wouter G.
_eco-author
856 _uhttps://doi.org/10.1016/j.jplph.2015.04.005
942 _2lcc
_cJA
999 _c169200
_d169200