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| 005 | 20250305073609.0 | ||
| 008 | 250305b |||||||| |||| 00| 0 eng d | ||
| 022 | _a0176-1617 | ||
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_aMSU _bEnglish _cMSU _erda |
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| 050 | 0 | 0 | _aQK711.2 JOU |
| 100 | 1 |
_aMittag, Jennifer _eauthor |
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| 245 | 1 | 0 |
_aPhyscomitrella patens auxin conjugate synthetase (GH3) double knockout mutants are more resistant to Pythium infection than wild type _ccreated by Jennifer Mittag, Ivana Šola, Gordana Rusak and Jutta Ludwig-Müller |
| 264 | 1 |
_aAmsterdam: _bElsevier GmbH, _c2015. |
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| 336 |
_2rdacontent _atext _btxt |
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| 337 |
_2rdamedia _aunmediated _bn |
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| 338 |
_2rdacarrier _avolume _bnc |
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| 440 |
_aJournal of plant physiology _vVolume 183 |
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| 520 | 3 | _aAuxin homeostasis is involved in many different plant developmental and stress responses. The auxin amino acid conjugate synthetases belonging to the GH3 family play major roles in the regulation of free indole-3-acetic acid (IAA) levels and the moss Physcomitrella patens has two GH3 genes in its genome. A role for IAA in several angiosperm – pathogen interactions was reported, however, in a moss – oomycete pathosystem it had not been published so far. Using GH3 double knockout lines we have investigated the role of auxin homeostasis during the infection of P. patens with the two oomycete species, Pythium debaryanum and Pythium irregulare. We show that infection with P. debaryanum caused stronger disease symptoms than with P. irregulare. Also, P. patens lines harboring fusion constructs of an auxin-inducible promoter from soybean (GmGH3) with a reporter (ß-glucuronidase) showed higher promoter induction after P. debaryanum infection than after P. irregulare, indicating a differential induction of the auxin response. Free IAA was induced upon P. debaryanum infection in wild type by 1.6-fold and in two GH3 double knockout (GH3-doKO) mutants by 4- to 5-fold. All GH3-doKO lines showed a reduced disease symptom progression compared to wild type. Since P. debaryanum can be inhibited in growth on medium containing IAA, these data might indicate that endogenous high auxin levels in P. patens GH3-doKO mutants lead to higher resistance against the oomycete. | |
| 650 | _aAuxin homeostasis | ||
| 650 | _aIndole-3-acetic acid | ||
| 650 | _aNon-vascular plant | ||
| 700 | 1 |
_aŠola, Ivana _eco-author |
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| 700 | 1 |
_aRusak, Gordana _eco-author |
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| 700 | 1 |
_aLudwig-Müller, Jutta _eco-author |
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| 856 | _uhttps://doi.org/10.1016/j.jplph.2015.05.015 | ||
| 942 |
_2lcc _cJA |
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_c169132 _d169132 |
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