| 000 | 02542nam a22002897a 4500 | ||
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| 003 | ZW-GwMSU | ||
| 005 | 20250304123709.0 | ||
| 008 | 250304b |||||||| |||| 00| 0 eng d | ||
| 022 | _a0176-1617 | ||
| 040 |
_aMSU _bEnglish _cMSU _erda |
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| 050 | _aQK711.2 JOU | ||
| 100 | 1 |
_aJeong, Ho Young _eauthor |
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| 245 | 1 | 0 |
_aGenome-wide identification and expression analysis of rice pectin methylesterases: _bimplication of functional roles of pectin modification in rice physiology _ccreated by Ho Young Jeong, Hong Phuong Nguyen, Chanhui Lee |
| 264 | 1 |
_aAmsterdam: _bElsevier GmbH, _c2015. |
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| 336 |
_2rdacontent _atext _btxt |
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| 337 |
_2rdamedia _aunmediated _bn |
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| 338 |
_2rdacarrier _avolume _bnc |
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| 440 |
_aJournal of plant physiology _vVolume 183 |
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| 520 | 3 | _aPectin, which is enriched in primary cell walls and middle lamellae, is an essential polysaccharide in all higher plants. Homogalacturonans (HGA), a major form of pectin, are synthesized and methylesterified by enzymes localized in the Golgi apparatus and transported into the cell wall. Depending on cell type, the degree and pattern of pectin methylesterification are strictly regulated by cell wall-localized pectin methylesterases (PMEs). Despite its importance in plant development and growth, little is known about the physiological functions of pectin in rice, which contains 43 different types of PME. The presence of pectin in rice cell walls has been substantiated by uronic acid quantification and immunodetection of JIM7 monoclonal antibodies. We performed PME activity assays with cell wall proteins isolated from different rice tissues. In accordance with data from Arabidopsis, the highest activity was observed in germinating tissues, young culm, and spikelets, where cells are actively elongating. Transcriptional profiling of OsPMEs by real-time PCR and meta-analysis indicates that PMEs exhibit spatial- and stress-specific expression patterns during rice development. Based on in silico analysis, we identified subcellular compartments, isoelectric point, and cleavage sites of OsPMEs. Our findings provide an important tool for further studies seeking to unravel the functional importance of pectin modification during plant growth and abiotic and biotic responses of grass plants. | |
| 650 | _aCell wall modifications | ||
| 650 | _aPectin | ||
| 650 | _aPectin methylesterases | ||
| 700 |
_aNguyen, Hong Phuong _eco-author |
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| 700 |
_aChanhui, Lee _eco-author |
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| 856 | _uhttps://doi.org/10.1016/j.jplph.2015.05.001 | ||
| 942 |
_2lcc _cJA |
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| 999 |
_c169118 _d169118 |
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