000 | 02686nam a22003617a 4500 | ||
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003 | ZW-GwMSU | ||
005 | 20250304122730.0 | ||
008 | 250304b |||||||| |||| 00| 0 eng d | ||
022 | _a0176-1617 | ||
040 |
_aMSU _bEnglish _cMSU _erda |
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050 | 0 | 0 | _aQK711.2 JOU |
100 | 1 |
_aDu, Ba _eauthor |
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245 | 1 | 0 |
_aPhloem-exudate proteome analysis of response to insect brown plant-hopper in rice _ccreated by Ba Du, Zhe Wei, Zhanqi Wang, Xiaoxiao Wang, Xinxin Peng, Bo Du, Rongzhi Chen, Lili Zhu and Guangcun He |
264 | 0 |
_aAmsterdam: _bElsevier GmbH, _c2015. |
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336 |
_2rdacontent _atext _btxt |
||
337 |
_2rdamedia _aunmediated _bn |
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338 |
_2rdacarrier _avolume _bnc |
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440 |
_aJournal of plant physiology _vVolume 183 |
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520 | 3 | _aBrown plant-hopper (Nilaparvata lugens Stål, BPH), one of the most devastating agricultural insect pests of rice throughout Asia, ingests nutrients from rice sieve tubes and causes a dramatic yield loss. Planting resistant variety is an efficient and economical way to control this pest. Understanding the mechanisms of host resistance is extremely valuable for molecular design of resistant rice variety. Here, we used an iTRAQ-based quantitative proteomics approach to perform analysis of protein expression profiles in the phloem exudates of BPH-resistant and susceptible rice plants following BPH infestation. A total of 238 proteins were identified, most of which were previously described to be present in the phloem of rice and other plants. The expression of genes for selected proteins was confirmed using a laser capture micro-dissection method and RT-PCR. The mRNAs for three proteins, RGAP, TCTP, and TRXH, were further analyzed by using in situ mRNA hybridization and localized in the phloem cells. Our results showed that BPH feeding induced significant changes in the abundance of proteins in phloem sap of rice involved in multiple pathways, including defense signal transduction, redox regulation, and carbohydrate and protein metabolism, as well as cell structural proteins. The results presented provide new insights into rice resistance mechanisms and should facilitate the breeding of novel elite BPH-resistant rice varieties. | |
650 | _aNilaparvata lugens | ||
650 | _aOryza sativa | ||
650 | _aPlant-insect interactions | ||
700 | 1 |
_aWei, Zhe _eco-author |
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700 | 1 |
_aWang, Zhanqi _eco-author |
|
700 | 1 |
_aWang, Xiaoxiao _eco-author |
|
700 | 1 |
_aPeng, Xinxin _eco-author |
|
700 | 1 |
_aDu, Bo _eco-author |
|
700 | 1 |
_aChen, Rongzhi _eco-author |
|
700 | 1 |
_aZhu, Lili _eco-author |
|
700 | 1 |
_aHe, Guangcun _eco-author |
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856 | _uhttps://doi.org/10.1016/j.jplph.2015.03.020 | ||
942 |
_2lcc _cJA |
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999 |
_c169117 _d169117 |