000 02686nam a22003617a 4500
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005 20250304122730.0
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022 _a0176-1617
040 _aMSU
_bEnglish
_cMSU
_erda
050 0 0 _aQK711.2 JOU
100 1 _aDu, Ba
_eauthor
245 1 0 _aPhloem-exudate proteome analysis of response to insect brown plant-hopper in rice
_ccreated by Ba Du, Zhe Wei, Zhanqi Wang, Xiaoxiao Wang, Xinxin Peng, Bo Du, Rongzhi Chen, Lili Zhu and Guangcun He
264 0 _aAmsterdam:
_bElsevier GmbH,
_c2015.
336 _2rdacontent
_atext
_btxt
337 _2rdamedia
_aunmediated
_bn
338 _2rdacarrier
_avolume
_bnc
440 _aJournal of plant physiology
_vVolume 183
520 3 _aBrown plant-hopper (Nilaparvata lugens Stål, BPH), one of the most devastating agricultural insect pests of rice throughout Asia, ingests nutrients from rice sieve tubes and causes a dramatic yield loss. Planting resistant variety is an efficient and economical way to control this pest. Understanding the mechanisms of host resistance is extremely valuable for molecular design of resistant rice variety. Here, we used an iTRAQ-based quantitative proteomics approach to perform analysis of protein expression profiles in the phloem exudates of BPH-resistant and susceptible rice plants following BPH infestation. A total of 238 proteins were identified, most of which were previously described to be present in the phloem of rice and other plants. The expression of genes for selected proteins was confirmed using a laser capture micro-dissection method and RT-PCR. The mRNAs for three proteins, RGAP, TCTP, and TRXH, were further analyzed by using in situ mRNA hybridization and localized in the phloem cells. Our results showed that BPH feeding induced significant changes in the abundance of proteins in phloem sap of rice involved in multiple pathways, including defense signal transduction, redox regulation, and carbohydrate and protein metabolism, as well as cell structural proteins. The results presented provide new insights into rice resistance mechanisms and should facilitate the breeding of novel elite BPH-resistant rice varieties.
650 _aNilaparvata lugens
650 _aOryza sativa
650 _aPlant-insect interactions
700 1 _aWei, Zhe
_eco-author
700 1 _aWang, Zhanqi
_eco-author
700 1 _aWang, Xiaoxiao
_eco-author
700 1 _aPeng, Xinxin
_eco-author
700 1 _aDu, Bo
_eco-author
700 1 _aChen, Rongzhi
_eco-author
700 1 _aZhu, Lili
_eco-author
700 1 _aHe, Guangcun
_eco-author
856 _uhttps://doi.org/10.1016/j.jplph.2015.03.020
942 _2lcc
_cJA
999 _c169117
_d169117