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| 005 | 20250304123946.0 | ||
| 008 | 250304b |||||||| |||| 00| 0 eng d | ||
| 022 | _a0176-1617 | ||
| 040 |
_aMSU _bEnglish _cMSU _erda |
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| 050 | 0 | 0 | _aQK711.2 JOU |
| 100 | 1 |
_aKubala, Szymon _eauthor |
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| 245 | 1 | 0 |
_aEnhanced expression of the proline synthesis gene P5CSA in relation to seed osmopriming improvement of Brassica napus germination under salinity stress _cSzymon Kubala, Łukasz Wojtyla, Muriel Quinet, Katarzyna Lechowska, Stanley Lutts and Małgorzata Garnczarska |
| 264 | 1 |
_aAmsterdam: _bElsevier GmbH, _c2015. |
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| 336 |
_2rdacontent _atext _btxt |
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| 337 |
_2rdamedia _aunmediated _bn |
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| 338 |
_2rdacarrier _avolume _bnc |
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| 440 |
_aJournal of plant physiology _vVolume 183 |
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| 520 | 3 | _aOsmopriming is a pre-sowing treatment that enhances germination performance and stress tolerance of germinating seeds. Brassica napus seeds showed osmopriming-improved germination and seedling growth under salinity stress. To understand the molecular and biochemical mechanisms of osmopriming-induced salinity tolerance, the accumulation of proline, gene expression and activity of enzymes involved in proline metabolism and the level of endogenous hydrogen peroxide were investigated in rape seeds during osmopriming and post-priming germination under control (H2O) and stress conditions (100 mM NaCl). The relationship between gene expression and enzymatic activity of pyrroline-5-carboxylate synthetase (P5CS), ornithine-δ-aminotransferase (OAT) and proline dehydrogenase (PDH) was determined. The improved germination performance of osmoprimed seeds was accompanied by a significant increase in proline content. The accumulation of proline during priming and post-priming germination was associated with strong up-regulation of the P5CSA gene, down-regulation of the PDH gene and accumulation of hydrogen peroxide. The up-regulated transcript level of P5CSA was consistent with the increase in P5CS activity. This study shows, for the first time, the role of priming-induced modulation of activities of particular genes and enzymes of proline turnover, and its relationship with higher content of hydrogen peroxide, in improving seed germination under salinity stress. Following initial stress-exposure, the primed seeds acquired stronger salinity stress tolerance during post-priming germination, a feature likely linked to a ‘priming memory’. | |
| 650 | _aGermination | ||
| 650 | _aOsmopriming | ||
| 650 | _aP5SCA | ||
| 700 | 1 |
_aWojtyla, Łukasz _eco-author |
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| 700 | 1 |
_aQuinet, Muriel _eco-author |
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| 700 | 1 |
_aLechowska, Katarzyna _eco-author |
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| 700 | 1 |
_aLutts, Stanley _eco-author |
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| 700 | 1 |
_aGarnczarska, Małgorzata _eco-author |
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| 856 | _uhttps://doi.org/10.1016/j.jplph.2015.04.009 | ||
| 942 |
_2lcc _cJA |
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| 999 |
_c169116 _d169116 |
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