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008			250304b |||||||| |||| 00| 0 eng d
022			_a0176-1617
040			_aMSU _bEnglsih _cMSU _erda
050	0	0	_aQK711.2 JOU
100	1		_aMaikova,Anna _eauthor
245	1	4	_aThe HSP70 chaperone machines of Chlamydomonas are induced by cold stress _ccreated by Anna Maikova, Zhanneta Zalutskaya,Tatiana Lapina and Elena Ermilova
264	1		_aAmsterdam: _bElsevier GmbH, _c2016.
336			_2rdacontent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_2rdacarrier _avolume _bnc
440			_aJournal of plant physiology _vVolume 204
520	3		_aThe responses of Chlamydomonas reinhardtii cells to low temperatures have not been extensively studied compared with other stresses. Like other organisms, this green alga has heat shock protein 70s (HSP70s) that are located in chloroplast, mitochondrion and cytosol. To test whether temperature downshifts affected HSP70s synthesis, we used real-time PCR and protein gel blot analysis. C. reinhardtii cells exposed to cold stress show increased HSP70s mRNA levels. Genes encoding other components of HSP70 chaperone machines (e.g. CGE1, CDJ1, HSP90C and HSP90A) are also up-regulated in response to decreased temperature. We demonstrated that the accumulation of all analyzed mRNA occur more slowly and with reduced amplitude in cells exposed to cold than in cells treated with heat. Furthermore, C. reinhardtii cells display the splicing of the CGE1 transcript that was dependent on low temperature. Finally, the transcription regulator of C. reinhardtii HSF1 is also cold-responsive, suggesting its role in the transcriptional regulation of HSP genes at low temperature.
650			_aChlamydomonas reinhardtii
650			_aCold stress
650			_aGene expression
700	1		_aZalutskaya, Zhanneta _eco-author
700	1		_aLapina, Tatiana _eco-author
700	1		_aErmilova, Elena _eco-author
856			_uhttps://doi.org/10.1016/j.jplph.2016.07.012
942			_2lcc _cJA
999			_c169109 _d169109