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_aMSU _bEnglish _cMSU _erda |
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| 050 | 0 | 0 | _aQK711.2 JOU |
| 100 | 1 |
_aBettini, Priscilla P. _eauthor |
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| 245 | 1 | 0 |
_aAgrobacterium rhizogenes rolB gene affects photosynthesis and chlorophyll content in transgenic tomato (Solanum lycopersicum L.) plants _ccreated byPriscilla P. Bettini, Massimiliano Marvasi, Fabiola Fani, Luigi Lazzara, Elena Cosi, Lorenzo Melani and Maria Luisa Mauro |
| 264 | 1 |
_aAmsterdam: _bElsevier GmbH, _c2016 |
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| 336 |
_2rdacontent _atext _btxt |
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| 337 |
_2rdamedia _aunmediated _bn |
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| 338 |
_2rdacarrier _avolume _bnc |
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| 440 |
_aJournal of plant physiology _vVolume 204 |
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| 520 | 3 | _aInsertion of Agrobacterium rhizogenes rolB gene into plant genome affects plant development, hormone balance and defence. However, beside the current research, the overall transcriptional response and gene expression of rolB as a modulator in plant is unknown. Transformed rolB tomato plant (Solanum lycopersicum L.) cultivar Tondino has been used to investigate the differential expression profile. Tomato is a well-known model organism both at the genetic and molecular level, and one of the most important commercial food crops in the world. Through the construction and characterization of a cDNA subtracted library, we have investigated the differential gene expression between transgenic clones of rolB and control tomato and have evaluated genes specifically transcribed in transgenic rolB plants. Among the selected genes, five genes encoding for chlorophyll a/b binding protein, carbonic anhydrase, cytochrome b6/f complex Fe-S subunit, potassium efflux antiporter 3, and chloroplast small heat-shock protein, all involved in chloroplast function, were identified. Measurement of photosynthesis efficiency by the level of three different photosynthetic parameters (Fv/Fm, rETR, NPQ) showed rolB significant increase in non-photochemical quenching and a, b chlorophyll content. Our results point to highlight the role of rolB on plant fitness by improving photosynthesis. | |
| 650 | _aAgrobacterium rhizogenes rolB gene | ||
| 650 | _aPhotosynthesis | ||
| 650 | _aSuppression subtractive hybridization | ||
| 700 | 1 |
_aMarvasi, Massimilian _eco-author |
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| 700 | 1 |
_aFani, Fabiola _eco-author |
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| 700 | 1 |
_aLazzara, Luigi _eco-author |
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| 700 | 1 |
_aCosi, Elena _eco-author |
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| 700 | 1 |
_aMelan, Lorenzo _eco-author |
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| 700 | 1 |
_aMauro, Maria Luisa _eco-author |
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| 856 | _uhttps://doi.org/10.1016/j.jplph.2016.07.010 Get rights and content | ||
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_2lcc _cJA |
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_c169081 _d169081 |
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