| 000 | 02441nam a22002897a 4500 | ||
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| 003 | ZW-GwMSU | ||
| 005 | 20250220104228.0 | ||
| 008 | 250220b |||||||| |||| 00| 0 eng d | ||
| 022 | _a0176-1617 | ||
| 040 |
_aMSU _bEnglish _cMSU _erda |
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| 050 | 0 | 0 | _aQK711.2 JOU |
| 100 | 1 |
_aFernandes, João C. _eauthor |
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| 245 | 1 | 0 |
_aRegulation of cell wall remodeling in grapevine (Vitis vinifera L.) callus under individual mineral stress deficiency _ccreated by João C. Fernandes, Luis F. Goulao and Sara Amâncio |
| 264 | 1 |
_aAmsterdam: _bElsevier GmbH, _c2016. |
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| 336 |
_2rdacontent _atext _btxt |
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| 337 |
_2rdamedia _aunmediated _bn |
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| 338 |
_2rdacarrier _avolume _bnc |
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| 440 |
_aJournal of plant physiology _vVolume 190 |
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| 520 | 3 | _aCell wall (CW) is a dynamic structure that determines the plant form, growth and response to environmental conditions. Vitis vinifera callus grown under nitrogen (−N), phosphorous (−P) and sulfur (−S) deficiency were used as a model system to address the influence of mineral stress in CW remodeling. Callus cells morphology was altered, mostly under –N, resulting in changes in cell length and width compared with the control. CW composition ascertained with specific staining and immuno-detection showed a decrease in cellulose and altered pattern of pectin methylesterification. Under mineral stress genes expression from candidate families disclosed mainly a downregulation of a glycosyl hydrolase family 9C (GH9C), xyloglucan transglycosylase/hydrolases (XTHs) with predicted hydrolytic activity and pectin methylesterases (PMEs). Conversely, upregulation of PMEs inhibitors (PMEIs) was observed. While methylesterification patterns can be associated to PME/PMEI gene expression, the lower cellulose content cannot be attributed to altered cellulose synthase (CesA) gene expression suggesting the involvement of other gene families. Salt extracts from –N and −P callus tissues increased plastic deformation in cucumber hypocotyls while no effect was observed with −S extracts. The lower endo-acting glycosyl hydrolase activity of −N callus extracts pinpoints a more expressive impact of −N on CW-remodeling. | |
| 650 | _aCell wall polymers | ||
| 650 | _aEndoglucanase activity | ||
| 650 | _aGene expression | ||
| 700 | 1 |
_aGoulao, Luis F. _eco-author |
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| 700 | 1 |
_aAmâncio, Sara _eco-author |
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| 856 | _uhttps://doi.org/10.1016/j.jplph.2015.10.007 | ||
| 942 |
_2lcc _cJA |
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| 999 |
_c169017 _d169017 |
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