000			02805nam a22003137a 4500
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008			250220b |||||||| |||| 00| 0 eng d
022			_a0176-1617
040			_aMSU _bEnglish _cMSU _erda
050	0	0	_aQK711.2 JOU
100	1		_aZhang, Qisen _eauthor
245	1	0	_aChanges in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos _ccreated by Qisen Zhang, Xiaoqi Zhang, Filomena Pettolino, Gaofeng Zhou and Chengdao Li
264	1		_aAmsterdam: _bElsevier GmbH, _c2016.
336			_2rdacontent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_2rdacarrier _avolume _bnc
440			_aJournal of plant physiology _vVolume 191
520	3		_aBarley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs. However, little is known on cell wall modifications in germinating embryos. We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24 h and 48 h germination. Cell wall components in germinating barley embryos changed rapidly, with increases in cellulose and (1,3)(1,4)-β-d-glucan (20–100%) within 24 h, but decreases in heteroxylan and arabinan (3–50%). There were also significant changes in the levels of type I arabinogalactans and heteromannans. Alternative splicing played very important roles in cell wall modifications. At least 22 cell wall transcripts were detected to undergo either alternative 3′ splicing, alternative 5′ splicing or intron retention type of alternative splicing. These genes coded enzymes catalyzing synthesis and degradation of cellulose, heteroxylan, (1,3)(1,4)-β-d-glucan and other cell wall polymers. Furthermore, transcriptional regulation also played very important roles in cell wall modifications. Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos. At least 50 cell wall genes changed transcript levels significantly. Expression patterns of many cell wall genes coincided with changes in polysaccharide composition. Our data showed that cell wall polysaccharide metabolism was very active in germinating barley embryos, which was regulated at both transcriptional and post-transcriptional levels.
650			_aAlternative splicing
650			_aCell wall
650			_aDifferentially expressed gene
700			_aZhang, Xiaoqi _eco-author
700			_aPettolino, Filomena _eco-author
700			_aZhou, Gaofeng _eco-author
700			_aLi, Chengdao _eco-author
856			_uhttps://doi.org/10.1016/j.jplph.2015.12.007
942			_2lcc _cJA
999			_c169004 _d169004