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_aMSU _bEnglish _cMSU _erda |
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| 050 | 0 | 0 | _aQK711.2 JOU |
| 100 | 1 |
_aVivancos, Julien _eauthor |
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| 245 | 1 | 0 |
_aIdentification and characterization of silicon efflux transporters in horsetail (Equisetum arvense) _ccreated by Julien Vivancos, Rupesh Deshmukh 1, Caroline Grégoire 1, Wilfried Rémus-Borel 1, François Belzile 2, Richard R Bélanger 3 |
| 264 | 1 |
_aAmsterdam: _bElsevier GmbH, _c2016. |
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| 336 |
_2rdacontent _atext _btxt |
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| 337 |
_2rdamedia _aunmediated _bn |
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| 338 |
_2rdacarrier _avolume _bnc |
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| 440 |
_aJournal of plant physiology _vVolume 200 |
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| 520 | 3 | _aSilicon (Si) is a beneficial element to plants, and its absorption via transporters leads to protective effects against biotic and abiotic stresses. In higher plants, two groups of root transporters for Si have been identified: influx transporters (Lsi1) and efflux transporters (Lsi2). Lsi1 transporters belong to the NIPIII aquaporins, and functional Lsi1s have been found in many plants species. Much less is known about Lsi2s that have been characterized in only a few species. Horsetail (Equisetum arvense), known among the highest Si accumulators in the plant kingdom, is a valuable model to study Si absorption and deposition. In this study, we first analyzed discrete Si deposition patterns in horsetail shoots, where ubiquitous silicification differs markedly from that of higher plants. Then, using the sequenced horsetail root transcriptome, two putative Si efflux transporter genes, EaLsi2-1 and EaLsi2-2, were identified. These genes share low sequence similarity with their homologues in higher plants. Further characterisation of EaLsi2-1 in transient expression assay using Nicotiana benthamiana epidermal cells confirmed transmembrane localization. In order to determine their functionality, the EaLsi2-1 was expressed in Xenopus oocytes, confirming that the translated protein was efficient for Si efflux. Both genes were equally expressed in roots and shoots, but interestingly, showed a much higher expression in the shoots than in the roots in contrast to Lsi2s found in other plants, a result consistent with the specific anatomy of horsetail and its rank as one of the highest Si accumulators among plant species. | |
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_aEfflux transporter _vEquisetum arvense _xLsi2 genes |
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_aDeshmukh, Rupesh _eco-author |
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| 700 |
_aGrégoire, Caroline _eco-author |
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| 700 |
_aRémus-Borel, Wilfried _eco-author |
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| 700 |
_aBelzile, François _eco-author |
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| 700 |
_aBélanger, Richard R _eco-author |
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| 856 | _uhttps://doi.org/10.1016/j.jplph.2016.06.011 | ||
| 942 |
_2lcc _cJA |
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_c168950 _d168950 |
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