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022 _a0176-1617
040 _aMSU
_bEnglish
_cMSU
_erda
050 0 0 _aQK711.2 JOU
100 1 _aMaschietto, Valentina
_eauthor
245 1 0 _aConstitutive expression of pathogenesis-related proteins and antioxydant enzyme activities triggers maize resistance towards Fusarium verticillioides
_ccreated by Valentina Maschietto, Alessandra Lanubile, Silvana De Leonardis, Adriano Marocco and Costantino Paciolla
264 1 _aAmsterdam:
_bElsevier GmbH,
_c2016.
336 _2rdacontent
_atext
_btxt
337 _2rdamedia
_aunmediated
_bn
338 _2rdacarrier
_avolume
_bnc
440 _aJournal of plant physiology
_vVolume 200
520 3 _aFusarium verticillioides is a fungal pathogen of maize that causes ear rot and contaminates the grains with fumonisin mycotoxins. Breeding for resistance to Fusarium emerged as the most economic and environmentally safe strategy; therefore the discovery of resistant sources and effective molecular markers are a priority. Ears of resistant (CO441 and CO433) and susceptible (CO354 and CO389) maize lines were inoculated with F. verticillioides and the expression of pathogenesis-related (PR) genes (PR1, PR5, PRm3, PRm6) and genes that protect from oxidative stress (peroxidase, catalase, superoxide dismutase and ascorbate peroxidase) were evaluated in the kernels at 72h post inoculation. In addition, the oxidation level and the enzymatic activity of ascorbate-glutathione cycle, catalase, superoxide dismutase and cytosolic and wall peroxidases were investigated. The uninoculated kernels of the resistant lines showed higher gene expression and enzymatic activities, highlighting the key role of constitutive resistance in limiting pathogen attack. In contrast, the susceptible lines activated defensive genes only after pathogen inoculation, resulting in increased levels of H2O2 and lipid peroxidation, as well as lower enzymatic activities. The constitutive defenses observed in this study from seed could be profitably exploited to develop markers to speed up conventional breeding programs in the selection of resistant genotypes.
650 _aDefense systems
_vFusarium verticillioides
_xOxidative stress
700 1 _aLanubile, Alessandra
_eco-author
700 1 _aDe Leonardis, Silvana
_eco-author
700 1 _aMarocco, Adriano
_eco-author
700 1 _aPaciolla, Costantino
_eco-author
856 _uhttps://doi.org/10.1016/j.jplph.2016.06.006
942 _2lcc
_cJA
999 _c168947
_d168947