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008			241216b |||||||| |||| 00| 0 eng d
022			_a0176-1617
040			_aMSU _bEnglish _cMSU _erda
050	0	0	_aQK711.2 JOU
100	1		_aCzégény, Gyula _eauthor
245	1	0	_aElevated ROS-scavenging enzymes contribute to acclimation to UV-B exposure in transplastomic tobacco plants, reducing the role of plastid peroxidases _ccreated by Gyula Czégény, Bénédicte Le Martret, Dóra Pávkovics, Philip J Dix and Éva Hideg
264	1		_aAmsterdam: _bElsevier GmbH, _c2016.
336			_2rdacontent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_2rdacarrier _avolume _bnc
440			_aJournal of plant physiology _vVolume 201
520	3		_aLeaf peroxidases play a key role in the successful acclimation of plants to low UV-B doses. The aim of the present study was to examine whether selective enhancement of alternative chloroplast antioxidant pathways achieved by chloroplast transformation affected the need for peroxidase defense. Transplastomic tobacco lines expressing glutathione reductase in combination with either dehydroascorbate reductase or glutathione-S-transferase in their plastids exhibited better tolerance to supplemental UV-B than wild type plants. After 10days UV treatment, both the maximum and effective quantum yields of PSII decreased in the wild type by 10% but were unaffected in either of the transformed lines. Activities of total peroxidase and ascorbate peroxidase, in addition to dehydroascorbate reductase and gluthatione-S-transferase, were increased by UV in all lines. Gluthatione reductase activity was unaffected by UV in the transplastomic line engineered to have a higher constitutive level of this enzyme, but increased in the two other genotypes. However, the observed more successful acclimation required less activation of peroxidases in the doubly transformed plants than in the wild type and less increase in non-enzymatic hydroxyl radical neutralization in the dehydroascorbate reductase plus glutathione reductase fortified plants than in either of the other lines. These results highlight the fundamental role of efficient glutathione, and especially ascorbate, recycling in the chloroplast in response to exposure of plants to UV-B. They also identify chloroplast localized peroxidases among the large variety of leaf peroxidases as essential elements of defense, supporting our earlier hypothesis on hydrogen peroxide UV-B photo-cleavage as the primary mechanism behind damage.
650			_aChloroplast transformant _vDehydroascorbate reductase _xGlutathione reductase
700			_aLe Martret, Bénédicte _eauthor
700			_aPávkovics, Dóra _eauthor
700			_aDix, Philip J _eauthor
700			_aHideg, Éva _eauthor
856			_uhttps://doi.org/10.1016/j.jplph.2016.05.026
942			_2lcc _cJA
999			_c168905 _d168905