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| 005 | 20241216093706.0 | ||
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| 040 |
_aMSU _bEnglish _cMSU _erda |
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| 050 | 0 | 0 | _aQK711.2 JOU |
| 100 | 1 |
_aBonifacio, Aurenivia _eauthor |
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| 245 | 1 | 0 |
_aSilenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase _ccreated by Aurenivia Bonifacio, FabrÃcio E L Carvalho, Marcio O Martins 1, Milton C Lima Neto 2, Juliana R Cunha 1, Carolina W Ribeiro 3, Marcia Margis-Pinheiro 3, Joaquim A G Silveira |
| 264 | 1 |
_aAmsterdam: _bElsevier GmbH, _c2016. |
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| 336 |
_2rdacontent _atext _btxt |
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| 337 |
_2rdamedia _aunmediated _bn |
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| 338 |
_2rdacarrier _avolume _bnc |
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| 440 |
_aJournal of plant physiology _vVolume 201 |
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| 520 | 3 | _aThe maintenance of H2O2 homeostasis and signaling mechanisms in plant subcellular compartments is greatly dependent on cytosolic ascorbate peroxidases (APX1 and APX2) and peroxisomal catalase (CAT) activities. APX1/2 knockdown plants were utilized in this study to clarify the role of increased cytosolic H2O2 levels as a signal to trigger the antioxidant defense system against oxidative stress generated in peroxisomes after 3-aminotriazole-inhibited catalase (CAT). Before supplying 3-AT, silenced APX1/2 plants showed marked changes in their oxidative and antioxidant profiles in comparison to NT plants. After supplying 3-AT, APX1/2 plants triggered up-expression of genes belonging to APX (OsAPX7 and OsAPX8) and GPX families (OsGPX1, OsGPX2, OsGPX3 and OsGPX5), but to a lower extent than in NT plants. In addition, APX1/2 exhibited lower glycolate oxidase (GO) activity, higher CO2 assimilation, higher cellular integrity and higher oxidation of GSH, whereas the H2O2 and lipid peroxidation levels remained unchanged. This evidence indicates that redox pre-acclimation displayed by silenced rice contributed to coping with oxidative stress generated by 3-AT. We suggest that APX1/2 plants were able to trigger alternative oxidative and antioxidant mechanisms involving signaling by H2O2, allowing these plants to display effective physiological responses for protection against oxidative damage generated by 3-AT, compared to non-transformed plants. | |
| 650 |
_aAscorbate peroxidase _vHydrogen peroxide _xOryza sativa |
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| 700 | 1 |
_aCarvalho, FabrÃcio E L _eco-author |
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| 700 | 1 |
_aMartins, Marcio O _eco-author |
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| 700 | 1 |
_aNeto, Milton C Lima _eco-author |
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| 700 | 1 |
_aCunha, Juliana R _eco-author |
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| 700 | 1 |
_aRibeiro, Carolina W _eco-author |
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| 700 | 1 |
_aMargis-Pinheiro, Marcia _eco-author |
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| 700 | 1 |
_aSilveira, Joaquim A G _eco-author |
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| 856 | _u10.1016/j.jplph.2016.06.015 | ||
| 942 |
_2lcc _cJA |
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| 999 |
_c168891 _d168891 |
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