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022 _a0176-1617
040 _aMSU
_bEnglish
_cMSU
_erda
050 0 0 _aQK711.2 JOU
100 1 _aDalir, Neda
_eauthor
245 1 0 _aRoot uptake and translocation of nickel in wheat as affected by histidine
_ccreated by Neda Dalir and Amir Hossein Khoshgoftarmanesh
264 1 _aAmsterdam:
_bElsevier GmbH,
_c2015.
336 _2rdacontent
_atext
_btxt
337 _2rdamedia
_aunmediated
_bn
338 _2rdacarrier
_avolume
_bnc
440 _aJournal of plant physiology
_vVolume 184
520 3 _aThe role of histidine (His) on root uptake, xylem loading and root to shoot transport of nickel (Ni) was investigated in a winter (Triticum aestivum cv. Back Cross) and a durum wheat (Triticum durum cv. Durum) cultivar. Seedlings were grown in a modified Johnson nutrient solution and exposed to 10 μM of Ni and 100 μM of histidine (His) as no His, Ni (10) + His (100) and Ni(His) in a 1:1 mole ratio (1:1) complex. In our study, the presence of vanadate (a metabolic inhibitor) resulted in a significant decrease of root Ni uptake, indicating that a part of Ni uptake by the plant root is energy-dependent. Addition of His significantly increased the Ni content in shoots and roots of both wheat cultivars. The data suggest that the Ni(His) is most likely to be taken up as a complex or receptors at the membrane are able to enhance Ni uptake from Ni(His) complex. This result was indirectly supported by using EDTA as a strong chelating reagent to reduce the uptake of Ni(His) complexes. By using this ligand, the xylem loading of Ni and His was disproportionately reduced. Cycloheximide (a translation inhibitor) strongly decreased the release of His and Ni from the root into the xylem of wheat, suggesting the significance of a symplastic pathway for Ni loading into the xylem.
650 _aActive uptake
_vTriticum aestivum
_xTriticum durum
700 1 _aKhoshgoftarmanesh, Amir Hossein
_eco-author
856 _uhttps://doi.org/10.1016/j.jplph.2015.05.017
942 _2lcc
_cJA
999 _c168862
_d168862