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022 _a00218596
040 _aMSU
_bEnglish
_cMSU
_erda
050 0 0 _aS3 JOU
100 1 _aKrawutschke, M.
_eauthor
245 1 4 _aThe effect of cultivar on the changes in protein quality during wilting and ensiling of red clover (Trifolium pratense L.)/
_ccreated by M. Krawutschke, N. Weiher, J. Thaysen, Ralf Loges, Friedhelm Taube and Martin Gierus
264 1 _aCambridge :
_bCambridge University Press,
_c2013.
336 _2rdacontent
_atext
_btxt
337 _2rdamedia
_aunmediated
_bn
338 _2rdacarrier
_avolume
_bnc
440 _aJournal of agricultural science
_vVolume 151, number 4,
520 3 _aRed clover (Trifolium pratense L.) silage usually contains lower contents of non-protein nitrogen (NPN) compared with other forage legumes. This is often attributed to the polyphenol oxidase (PPO) activity in red clover, although in most field studies the PPO activity was not measured. Therefore, a laboratory ensiling experiment with three red clover cultivars and one white clover cultivar as control grown in two management systems (with and without mechanical stress) over 2 consecutive years was conducted. Fresh, wilted and ensiled clover herbage was sampled at four cutting dates per year to determine the crude protein (CP) fractions according to the Cornell Net Carbohydrate and Protein System. The specific PPO activity was measured photometrically in fresh clover leaves. The content of CP fraction A (NPN) increased from fresh over wilted to ensiled clover herbage at the expense of the content of CP fraction B (true protein), irrespective of species, cultivar and year. The most important source of variation for all CP fractions and the calculated rumen-undegradable protein contents was generally the herbage condition, except for CP fraction C (unavailable protein). White clover silage consisted of higher contents of CP fraction A and lower contents of CP fraction B3 in CP compared with red clover silage. As a result, the calculated rumen-undegradable protein content of white clover silage was lower than that of all red clover cultivars. In conclusion, the extent of proteolysis during ensiling among the silages made from the herbage of different red clover cultivars was primarily influenced by the stage of maturity at harvesting and the degree of wilting at ensiling. The variation in specific PPO activity could not be related to the extent of proteolysis.
650 _aRed clover (Trifolium pratense L.)
_vCultivar
_xProtein quality
700 1 _aN. Weiher,
_eco author
700 1 _aThaysen, J.
_eco author
700 1 _aRalf Loges, Friedhelm
_eco author
700 1 _aTaube, Martin Gierus
_eco author
856 _uhttps://doi.org/10.1017/S0021859612000597
942 _2lcc
_cJA
999 _c168826
_d168826