Biomaterial control of therapeutic stem cells / created by Akon Higuchi.

Includes bibliography and index

1.1 Introduction
1.2 Stem Cells
1.3 The Extracellular Matrix
1.4 hPSC Culture on Biomaterials
1.5 hPSC Differentiation on Biomaterials
1.6 Biomaterials Control hPS Cell Differentiation Fate
1.7 Stem Cell Therapy Using Biomaterials
References
Chapter 2. Adult Stem Cell Culture on Extracellular Matrices and Natural Biopolymers
2.1 Introduction
2.2 Chemical and Biological Interactions of ECM Proteins and Stem Cells
2.3 Collagen
2.3.1 Collagen Type I Scaffold
2.3.2 Organic Hybrid Scaffold Made of Collagen Type I2.3.3 Scaffolds Using Collagen Type II and Type III
2.3.4 Hybrid Collagen Scaffold Using Inorganic Materials
2.3.5 Collagen Scaffolds Immobilized Antibody Targeting Stem Cells
2.3.6 Differentiation into Endoderm and Ectoderm Lineages Using Collagen Scaffolds
2.4 Gelatin
2.4.1 Gelatin Hydrogels and Scaffolds
2.4.2 Gelatin Hybrid Scaffolds
2.5 Laminin
2.6 Fibronectin
2.7 Vitronectin
2.8 Fibrin
2.9 Decellularized ECM
2.10 Biomaterials with ECM-mimicking Oligopeptides
2.10.1 MS Cell Differentiation on Self-assembled ECM-peptide Nanofibers2.10.2 Osteogenic Induction on ECM-peptide Immobilized Dishes and Scaffolds
2.10.3 Chondrogenic Induction on ECM-peptide Immobilized Dishes and Scaffolds
2.10.4 Neural Induction on ECM-peptide Immobilized Dishes and Scaffolds
2.11 Biomaterials with N-Cadherin Mimicking Oligopeptides
2.12 Conclusion and Future Perspective
References Chapter 3. Feeder-free and Xeno-free Culture of Human Pluripotent Stem Cells on Biomaterials
3.1 Introduction
3.2 Analysis of the Pluripotency of hPS Cells
3.3 2D Cultivation of hPS Cells on Biomaterials3.3.1 hPS Cell Cultivation on ECM-immobilized Surfaces in 2D
3.3.2 hPS Cell Cultivation on Oligopeptide-immobilized Surfaces in 2D
3.3.3 hPS Cell Cultivation on a Recombinant E-cadherin Surface in 2D
3.3.4 hPS Cell Cultivation on Biomaterials Immobilized with Polysaccharide in 2D
3.3.5 hPS Cell Cultivation on Synthetic Biomaterials in 2D
3.4 Three-dimensional Cultivation of hPS Cells on Biomaterials
3.4.1 The 3D Cultivation of hPS Cells on Microcarriers
3.4.2 The 3D Cultivation of hPS Cells Embedded in Hydrogels (Microcapsules)
3.5 hPS Cell Cultivation on PDL-coated Dishes with Small Molecules3.6 Conclusion and Future Perspectives
Acknowledgements
References
Chapter 4. Differentiation Fates of Human ES and iPS Cells Guided by Physical Cues of Biomaterials
4.1 Introduction
4.2 Induction Protocols of Human Pluripotent Stem Cells
4.2.1 EB Formation
4.2.2 Induction of hPS Cells by EB Generation
4.2.3 Induction of hPS Cells Seeded on Materials Directly
4.3 Physical Cues of Materials in hPS Cell Induction
4.3.1 Effect of Elasticity of Cell Cultivation Biomaterials on Stem Cell Induction
3.2 Topographic Effects of Biomaterials on the Differentiation Fates of hPS Cells
4.3.3 Stem Cell Induction on Nanofibers
4.3.4 Effect of Electrical and Mechanical
Forces of Biomaterials on Induction Fate of hPS Cells
4.4 Conclusions and Perspectives
References Chapter 5. Biomaterial Control of Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
5.1 Introduction
5.2 Induction of hPS Cells into Neural Lineages
5.2.1 Stromal-induced Differentiation into Neural Lineages
5.2.2 Induction into Neural Lineages Through EB Generation
5.2.3 Direct Induction into Neural Lineages on Materials with No EB Generation
5.2.4 Effect of Cell Cultivation Materials on hPS Cell Induction into Neural Lineages
5.3 Induction of hPS Cells into Cardiomyocytes
5.3.1 Efficient Protocols for Inducing hPS
Cells into Cardiomyocyte
5.3.2 Effect of Cell Cultivation Materials on hPS Cell Induction into Cardiomyocytes
5.4 Induction into Hepatocytes
5.4.1 Efficient Protocols for hPS Cell Induction into Hepatocytes on Materials
5.4.2 3D Cultivation Facilitates the Induction of hPS Cells into Hepatocytes
5.4.3 Effect of Cell Culture Biomaterials on hPS Cell Differentiation into Hepatocytes
5.5 Differentiation into Insulin-secreting b Cells
5.6 Conclusions and Perspectives
References Chapter 6. Clinical Trials of Stem Cell Therapies Using Biomaterials
6.1 Introduction
6.2 Stem Cell Therapy for Myocardial Infarction (MI) in Clinical Trials
6.2.1 Clinical Therapies for MI Using hES cells
6.2.2 Clinical Therapy for MI Using Fetal and Adult Stem Cells
6.2.3 Future Trends of MI Therapy Using Stem Cells
6.3 Stem Cell Therapy for Macular Degeneration Disease in Clinical Trials
6.3.1 Macular Degeneration Diseases and Eye Structure
6.3.2 Bioengineering in Stem Cell Therapies for Macular Degeneration Diseases
6.3.3 Biomaterial Assists in the Therapies for Macular Degeneration Diseases
6.3.4 Bioengineering for Clinical Trials Using hES Cell-derived RPE Cells
6.3.5 Bioengineering for Clinical Trials Using hiPS Cell-derived RPE Sheets
6.3.6 Bioengineering for Clinical Trials Using Adult Stem Cells
6.3.7 Clinical Trials Using Fetal Stem Cells
6.3.8 Future Perspectives of Stem Cell Therapy for Macular Degeneration Diseases
References
Chapter 7. Conclusions and Future Perspective on Biomaterial Control of Therapeutic Stem Cells

Using this book, the reader will gain a robust overview of current research and a clearer understanding of the status of clinical trials for stem cell therapies