TY  - BOOK
AU  - Uji,Yuya
AU  - Ozawa,Rika
AU  - Shishido,Hodaka
AU  - Taniguchi,Shiduku
AU  - Takabayashi,Junji
AU  - Akimitsu,Kazuya
AU  - Gomi,Kenji
TI  - Isolation of a sesquiterpene synthase expressing in specialized epithelial cells surrounding the secretory cavities in rough lemon (Citrus jambhiri)
SN  - 0176-1617
AV  - QK711.2 JOU 
PY  - 2015///
CY  - Amsterdam
PB  - Elsevier GmbH
KW  - Citrus
KW  - Essential oil
KW  - Sesquiterpene
N2  - Volatile terpenoids such as monoterpenes and sesquiterpenes play multiple roles in plant responses and are synthesized by terpene synthases (TPSs). We have previously isolated a partial TPS gene, RlemTPS4, that responds to microbial attack in rough lemon. In this study, we isolated a full length RlemTPS4 cDNA from rough lemon. RlemTPS4 localized in the cytosol. The recombinant RlemTPS4 protein was obtained using a prokaryotic expression system and GC–MS analysis of the terpenes produced by the RlemTPS4 enzymatic reaction determined that RlemTPS4 produces some sesquiterpenes such as δ-elemene. The RlemTPS4 gene was specifically expressed in specialized epithelial cells surrounding the oil secretory cavities in rough lemon leaf tissue
UR  - https://doi.org/10.1016/j.jplph.2015.03.013
ER  -