TY - BOOK AU - Jain,Akansha AU - Singh,Akanksha AU - Singh,Surendra AU - Singh,Vinay AU - Singh,Harikesh Bahadur TI - Comparative proteomic analysis in pea treated with microbial consortia of beneficial microbes reveals changes in the protein network to enhance resistance against Sclerotinia sclerotiorum SN - 0176-1617 AV - QK711.2 JOU PY - 2015/// CY - Amsterdam PB - Elsevier GmbH KW - Bacillus subtilis KW - Microbial consortium KW - Pseudomonas aeruginosa N2 - Microbial consortia may provide protection against pathogenic ingress via enhancing plant defense responses. Pseudomonas aeruginosa PJHU15, Trichoderma harzianum TNHU27 and Bacillus subtilis BHHU100 were used either singly or in consortia in the pea rhizosphere to observe proteome level changes upon Sclerotinia sclerotiorum challenge. Thirty proteins were found to increase or decrease differentially in 2-DE gels of pea leaves, out of which 25 were identified by MALDI-TOF MS or MS/MS. These proteins were classified into several functional categories including photosynthesis, respiration, phenylpropanoid metabolism, protein synthesis, stress regulation, carbohydrate and nitrogen metabolism and disease/defense-related processes. The respective homologue of each protein identified was trapped in Pisum sativum and a phylogenetic tree was constructed to check the ancestry. The proteomic view of the defense response to S. sclerotiorum in pea, in the presence of beneficial microbes, highlights the enhanced protection that can be provided by these microbes in challenged plants UR - https://doi.org/10.1016/j.jplph.2015.05.004 ER -