TY  - BOOK
AU  - Rani,T Swaroopa
AU  - Durgeshwar,P.
AU  - Podile,Appa Rao
TI  - Accumulation of transcription factors and cell signaling-related proteins in the nucleus during citrus-Xanthomonas interaction
SN  - 0176-1617
AV  - QK711.2 JOU 
PY  - 2015///
CY  - Amsterdam
PB  - Elsevier GmbH
KW  - Citrus
KW  - 2DE
KW  - Nano-LC MS/MS
N2  - The nucleus is the maestro of the cell and is involved in the modulation of cell signaling during stress. We performed a comprehensive nuclear proteome analysis of Citrus sinensis during interaction with host (Xanthomonas citri pv. citri-Xcc) and non-host (Xanthomonas oryzae pv. oryzae-Xoo) pathogens. The nuclear proteome was obtained using a sequential method of organelle enrichment and determined by nano-LC-MS/MS analysis. A total of 243 proteins accumulated differentially during citrus-Xanthomonas interaction, belonging to 11 functional groups, with signaling and transcription-related proteins dominating. MADS-box transcription factors, DEAD-box RNA helicase and leucine aminopeptidase, mainly involved in jasmonic acid (JA) responses, were in high abundance during non-host interaction (Xoo). Signaling-related proteins like serine/threonine kinase, histones (H3.2, H2A), phosphoglycerate kinase, dynamin, actin and aldolase showed increased accumulation early during Xoo interaction. Our results suggest that there is a possible involvement of JA-triggered defense responses during non-host resistance, with early recognition of the non-host pathogen
UR  - https://doi.org/10.1016/j.jplph.2015.05.013
ER  -