Bioconjugate techniques / created by Greg T. Hermanson, Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL.

Includes bibliographical references (pages 1017-1094) and index.

Introduction to bioconjugation Functional targets for bioconjugation The reactions of bioconjugation Zero-length crosslinkers Homobifunctional crosslinkers Heterobifunctional crosslinkers Trifunctional crosslinkers Dendrimers and dendrons Cross-bridges and cleavable reagent systems Fluorescent probes (Strept)avidin-biotin systems Isotopic labeling techniques Silane coupling agents Microparticles and nanoparticles Immobilization of ligands on chromatography supports Buckyballs, fullerenes, and carbon nanotubes Chemoselective ligation; bioorthogonal reagents Pegylation and synthetic polymer modification Vaccines and immunogen conjugates Antibody modification and conjugation Liposome conjugates and derivatives Enzyme modification and conjugation Nucleic acid and oligonucleotide modification and conjugation Bioconjugation in the study of protein interactions

Bioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. *Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab *Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates *Features full color illustrations *Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented