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Protocols for macroalgae research / created by Bénédicte Charrier, Thomas Wichard and CRK Reddy.

By: Contributor(s): Material type: TextTextPublisher: Taylor & Francis, 2017Copyright date: ©2018Description: xxii, 491 pages coloured illustrations; 25 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
ISBN:
  • 9781498796422 (hardback : alk. paper)
Subject(s): LOC classification:
  • QK570.2 PRO
Contents:
Cover; Half Title; Title Page; Copyright Page; Table of Contents; Preface; Editors; Contributors; Section I: Cultivating and preserving seaweeds; Chapter 1: Seaweed in high-energy environments: Protocol to move Saccharina cultivation offshore; Chapter 2: Cultivation protocol for Saccharina latissima; Chapter 3: Derivation of clonal stock cultures and hybridization of kelps: A tool for strain preservation and breeding programs; Chapter 4: Cryopreservation of macroalgae; Chapter 5: Unraveling seaweeds bacteriomes: From field site to computer screen. Chapter 6: Heavy metal ecotoxicity on the early life history stages of macroalgaeChapter 7: A simple protocol for a rapid and consistent production of a large number of viable protoplasts from the Ulvophycean species; Chapter 8: Purification of sporulation and swarming inhibitors from Ulva: Application in algal life-cycle controlling; Chapter 9: Preparation of axenic cultures in Ulva (Chlorophyta); Section II: Chemical composition; Chapter 10: Biochar production from seaweeds; Chapter 11: Identification and quantification of laminarins in brown algae. Chapter 12: Determination of carbohydrate composition of macroalgae Chapter 13: Quantification of proteins in seaweeds; Chapter 14: Comprehensive phytohormone quantification in the red alga Pyropia yezoensis by liquid chromatography-mass spectrometry; Chapter 15: Total phenolic content and antioxidant capacity analysis of seaweed biomass; Chapter 16: Extraction of phycocyanin and phycoerythrin pigments; Chapter 17: Quantification and localization of reactive oxygen species in marine macrophytes. Chapter 18: Metabolomics of intra- and extracellular metabolites from micro- and macroalgae using GC-MS and LC-MS Chapter 19: Preparative extraction of exometabolites from seaweed surfaces; Chapter 20: Disruption-free solid-phase extraction of surface metabolites from macroalgae; Section III: Cellular and molecular characterization; Chapter 21: The immunodetection and in situ imaging of cell-wall polysaccharides in brown algae; Chapter 22: Atomic force microscopy based analysis of cell-wall elasticity in macroalgae. Chapter 23: Dynamic and microscale mapping of cell growth: Case of Ectocarpus filament cellsChapter 24: Actin fluorescent staining in the filamentous brown alga Ectocarpus siliculosus; Chapter 25: Cryofixation of brown algae for transmission electron microscopy; Chapter 26: Probing the subcellular topography of seaweeds: Transmission electron microscopy, immunocytochemistry, and correlative light microscopy; Chapter 27: Coralline algae preparation for scanning electron microscopy and optical microscopy; Chapter 28: Extraction of high quality RNA from brown algae for transcriptomic analysis
Summary: "This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research. A major focus will be on bioenergy related aspects of this emerging technology. These protocols will be written in a clear and concise manner using simple language permitting even nonspecialist to adequately understand the significance of this research. It will also contain all necesssary notes and guidelines for successful execution of these experiments."--Provided by publisher
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Holdings
Item type Current library Call number Copy number Status Date due Barcode
Book Book Main Library Open Shelf QK570.2 PRO (Browse shelf(Opens below)) 158418 Available BK145877

Includes bibliographical references.

Cover; Half Title; Title Page; Copyright Page; Table of Contents; Preface; Editors; Contributors; Section I: Cultivating and preserving seaweeds; Chapter 1: Seaweed in high-energy environments: Protocol to move Saccharina cultivation offshore; Chapter 2: Cultivation protocol for Saccharina latissima; Chapter 3: Derivation of clonal stock cultures and hybridization of kelps: A tool for strain preservation and breeding programs; Chapter 4: Cryopreservation of macroalgae; Chapter 5: Unraveling seaweeds bacteriomes: From field site to computer screen. Chapter 6: Heavy metal ecotoxicity on the early life history stages of macroalgaeChapter 7: A simple protocol for a rapid and consistent production of a large number of viable protoplasts from the Ulvophycean species; Chapter 8: Purification of sporulation and swarming inhibitors from Ulva: Application in algal life-cycle controlling; Chapter 9: Preparation of axenic cultures in Ulva (Chlorophyta); Section II: Chemical composition; Chapter 10: Biochar production from seaweeds; Chapter 11: Identification and quantification of laminarins in brown algae. Chapter 12: Determination of carbohydrate composition of macroalgae Chapter 13: Quantification of proteins in seaweeds; Chapter 14: Comprehensive phytohormone quantification in the red alga Pyropia yezoensis by liquid chromatography-mass spectrometry; Chapter 15: Total phenolic content and antioxidant capacity analysis of seaweed biomass; Chapter 16: Extraction of phycocyanin and phycoerythrin pigments; Chapter 17: Quantification and localization of reactive oxygen species in marine macrophytes. Chapter 18: Metabolomics of intra- and extracellular metabolites from micro- and macroalgae using GC-MS and LC-MS Chapter 19: Preparative extraction of exometabolites from seaweed surfaces; Chapter 20: Disruption-free solid-phase extraction of surface metabolites from macroalgae; Section III: Cellular and molecular characterization; Chapter 21: The immunodetection and in situ imaging of cell-wall polysaccharides in brown algae; Chapter 22: Atomic force microscopy based analysis of cell-wall elasticity in macroalgae. Chapter 23: Dynamic and microscale mapping of cell growth: Case of Ectocarpus filament cellsChapter 24: Actin fluorescent staining in the filamentous brown alga Ectocarpus siliculosus; Chapter 25: Cryofixation of brown algae for transmission electron microscopy; Chapter 26: Probing the subcellular topography of seaweeds: Transmission electron microscopy, immunocytochemistry, and correlative light microscopy; Chapter 27: Coralline algae preparation for scanning electron microscopy and optical microscopy; Chapter 28: Extraction of high quality RNA from brown algae for transcriptomic analysis

"This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research. A major focus will be on bioenergy related aspects of this emerging technology. These protocols will be written in a clear and concise manner using simple language permitting even nonspecialist to adequately understand the significance of this research. It will also contain all necesssary notes and guidelines for successful execution of these experiments."--Provided by publisher

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