Acute toxicity test using cyanide on Daphnia magna by flow-through system

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JAAFARZADEH, N

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Material type: Text

TextSeries: Journal of Water Chemistry and Technology ; Volume , number ,Kiev Allerton Press 2013Content type:

text

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unmediated

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volume

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Summary: This study describes the acute toxicity of sodium cyanide on the crustacean Daphnia magna (Cladocera). A new flow-through system was innovated in which the microorganism continuously exposure to the toxicity of sodium cyanide during the test. We used twelve different concentration of sodium cyanide from low values (0 mg CN/L) to high values (1 mg CN/L) on bioassay test. D. magna were exposed to the concentrations for 24, 48, 72 and 96 h at 20–25°C. We controlled the three important parameters such as temperature, pH and DO to meet the standard requirements. The LC50 values for 24, 48, 72 and 96 h (95% confidence limits in parentheses) were estimated statistically by the probit methods and were 0.171 (0.163–0.179), 0.12 (0.112–0.128), 0.07 (0.062–0.078) and 0.019 (0.011–0.027) mg/L respectively. Finally, we proposed two new values for SAR (safe application rate) and SAFE Coefficients.

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Item type	Current library	Call number	Vol info	Status	Notes	Date due	Barcode
Journal Article	Main Library - Special Collections	GB855 JOU (Browse shelf(Opens below))	Vol.35 , No. 6 (Nov - Dec 2013)	Not for loan	For In House Use Only

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This study describes the acute toxicity of sodium cyanide on the crustacean Daphnia magna (Cladocera). A new flow-through system was innovated in which the microorganism continuously exposure to the toxicity of sodium cyanide during the test. We used twelve different concentration of sodium cyanide from low values (0 mg CN/L) to high values (1 mg CN/L) on bioassay test. D. magna were exposed to the concentrations for 24, 48, 72 and 96 h at 20–25°C. We controlled the three important parameters such as temperature, pH and DO to meet the standard requirements. The LC50 values for 24, 48, 72 and 96 h (95% confidence limits in parentheses) were estimated statistically by the probit methods and were 0.171 (0.163–0.179), 0.12 (0.112–0.128), 0.07 (0.062–0.078) and 0.019 (0.011–0.027) mg/L respectively. Finally, we proposed two new values for SAR (safe application rate) and SAFE Coefficients.

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