Midlands State University Library

Characterisation of the membrane transport of pilocarpine in cell suspension cultures of Pilocarpus microphyllus (Record no. 169307)

MARC details
000 -LEADER
fixed length control field 02733nam a22003017a 4500
003 - CONTROL NUMBER IDENTIFIER
control field ZW-GwMSU
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250317094245.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250317b |||||||| |||| 00| 0 eng d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 0176-1617
040 ## - CATALOGING SOURCE
Original cataloging agency MSU
Language of cataloging English
Transcribing agency MSU
Description conventions rda
050 00 - LIBRARY OF CONGRESS CALL NUMBER
Classification number QK711.2 JOU
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Andreazza, Nathalia Luiza
Relator term author
245 11 - TITLE STATEMENT
Title Characterisation of the membrane transport of pilocarpine in cell suspension cultures of Pilocarpus microphyllus
Statement of responsibility, etc. created by Nathalia Luiza Andreazza, Ilka Nacif Abreu, Alexandra Christine Helena Frankland Sawaya and Paulo Mazzafera
264 1# - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture Amsterdam:
Name of producer, publisher, distributor, manufacturer Elsevier GmbH,
Date of production, publication, distribution, manufacture, or copyright notice 2015.
336 ## - CONTENT TYPE
Source rdacontent
Content type term text
Content type code txt
337 ## - MEDIA TYPE
Source rdamedia
Media type term unmediated
Media type code n
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term volume
Carrier type code nc
440 ## - SERIES STATEMENT/ADDED ENTRY--TITLE
Title Journal of Plant Physiology,
Volume/sequential designation Volume 175
520 3# - SUMMARY, ETC.
Summary, etc. Pilocarpine is an alkaloid obtained from the leaves of Pilocarpus genus, with important pharmaceutical applications. Previous reports have investigated the production of pilocarpine by Pilocarpus microphyllus cell cultures and tried to establish the alkaloid biosynthetic route. However, the site of pilocarpine accumulation inside of the cell and its exchange to the medium culture is still unknown. Therefore, the aim of this study was to determine the intracellular accumulation of pilocarpine and characterise its transport across membranes in cell suspension cultures of P. microphyllus. Histochemical analysis and toxicity assays indicated that pilocarpine is most likely stored in the vacuoles probably to avoid cell toxicity. Assays with exogenous pilocarpine supplementation to the culture medium showed that the alkaloid is promptly uptaken but it is rapidly metabolised. Treatment with specific ABC protein transporter inhibitors and substances that disturb the activity of secondary active transporters suppressed pilocarpine uptake and release suggesting that both proteins may participate in the traffic of pilocarpine to inside and outside of the cells. As bafilomicin A1, a specific V-type ATPase inhibitor, had little effect and NH4Cl (induces membrane proton gradient dissipation) had moderate effect, while cyclosporin A and nifedipine (ABC proteins inhibitors) strongly inhibited the transport of pilocarpine, it is believed that ABC proteins play a major role in the alkaloid transport across membranes but it is not the exclusive one. Kinetic studies supported these results.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ABC proteins
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Pilocarpine
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Subcellular localisation
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Abreu, Ilka Nacif
Relator term co-author
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Sawaya, Alexandra Christine Helena Frankland
Relator term co-author
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Mazzafera, Paulo
Relator term co-author
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier https://doi.org/10.1016/j.jplph.2014.11.002
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Journal Article
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Shelving location Date acquired Serial Enumeration / chronology Total Checkouts Full call number Date last seen Price effective from Koha item type Public note
    Library of Congress Classification     Main Library Main Library - Special Collections 02/10/2015 Vol.175 (pages37-47)   QK711.2 JOU 17/03/2025 17/03/2025 Journal Article For in house use only