Midlands State University Library

Identification and characterization of a gene encoding for a nucleotidase from Phaseolus vulgaris (Record no. 168977)

MARC details
000 -LEADER
fixed length control field 02538nam a22003137a 4500
003 - CONTROL NUMBER IDENTIFIER
control field ZW-GwMSU
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20241218135831.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 241218b |||||||| |||| 00| 0 eng d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 0176-1617
040 ## - CATALOGING SOURCE
Original cataloging agency MSU
Language of cataloging English
Transcribing agency MSU
Description conventions rda
050 00 - LIBRARY OF CONGRESS CALL NUMBER
Classification number QK711.2 JOU
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Cabello-Díaz, Juan Miguel
Relator term author
245 10 - TITLE STATEMENT
Title Identification and characterization of a gene encoding for a nucleotidase from Phaseolus vulgaris
Statement of responsibility, etc. created by Juan Miguel Cabello-Díaz, Gregorio Gálvez-Valdivieso, Cristina Caballo, Rocío Lambert, Francisco Antonio Quiles ,Manuel Pineda, Pedro Piedras
264 1# - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture Amsterdam:
Name of producer, publisher, distributor, manufacturer Elsevier GmbH,
Date of production, publication, distribution, manufacture, or copyright notice 2016.
336 ## - CONTENT TYPE
Source rdacontent
Content type term text
Content type code txt
337 ## - MEDIA TYPE
Source rdamedia
Media type term unmediated
Media type code n
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term volume
Carrier type code nc
440 ## - SERIES STATEMENT/ADDED ENTRY--TITLE
Title Journal of plant physiology
Volume/sequential designation Volume 185
520 3# - SUMMARY, ETC.
Summary, etc. Nucleotidases are phosphatases that catalyze the removal of phosphate from nucleotides, compounds with an important role in plant metabolism. A phosphatase enzyme, with high affinity for nucleotides monophosphate previously identified and purified in embryonic axes from French bean, has been analyzed by MALDI TOF/TOF and two internal peptides have been obtained. The information of these peptide sequences has been used to search in the genome database and only a candidate gene that encodes for the phosphatase was identified (PvNTD1). The putative protein contains the conserved domains (motif I–IV) for haloacid dehalogenase-like hydrolases superfamily. The residues involved in the catalytic activity are also conserved. A recombinant protein overexpressed in Escherichia coli has shown molybdate resistant phosphatase activity with nucleosides monophosphate as substrate, confirming that the identified gene encodes for the phosphatase with high affinity for nucleotides purified in French bean embryonic axes. The activity of the purified protein was inhibited by adenosine. The expression of PvNTD1 gene was induced at the specific moment of radicle protrusion in embryonic axes. The gene was also highly expressed in young leaves whereas the level of expression in mature tissues was minimal.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Germination
Form subdivision Nucleoside
General subdivision Nucleotidase
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Gálvez-Valdivieso, Gregorio
Relator term co-author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Caballo, Cristina
Relator term co-author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Lambert, Rocío
Relator term co-author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Quiles, Francisco Antonio
Relator term co-author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Pineda, Manuel
Relator term co-author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Piedras, Pedro
Relator term co-author
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier https://doi.org/10.1016/j.jplph.2015.07.008
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Journal Article
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Shelving location Date acquired Serial Enumeration / chronology Total Checkouts Full call number Date last seen Price effective from Koha item type Public note
    Library of Congress Classification     Main Library Main Library - Special Collections 09/11/2015 Vol. 185 (pages44-51)   QK711.2 JOU 18/12/2024 18/12/2024 Journal Article For in house use only