Tubulin marker line of grapevine suspension cells as a tool to follow early stress responses
Guan, Xin
Tubulin marker line of grapevine suspension cells as a tool to follow early stress responses created by Xin Guan, Günther Buchholz and Peter Nick - Journal of plant physiology Volume 176 .
Plant microtubules (MTs), in addition to their role in cell division and cell expansion, respond to various stress signals. To understand the biological function of this early response requires non-destructive strategies for visualization in cellular models that are highly responsive to stress signals. We have therefore generated a transgenic tubulin marker line for a cell line from the grapevine Vitis rupestris that readily responds to stress factors of defense-related and abiotic stresses based on a fusion of the green fluorescent protein with Arabidopsis β-tubulin 6. By a combination of spinning-disk confocal microscopy with quantitative image analysis, we could detect early and specific responses of MTs to defense-related and abiotic stress factors in vivo. We observed that Harpin Z (HrpZ), a bacterial elicitor that can trigger programmed cell death, rapidly eliminated radial MTs, followed by a slower depletion of the cortical array. Jasmonic acid (JA), in contrast, induced bundling of cortical MTs. Auxin reduced the thickness of cortical MTs. This effect followed a characteristic bell-shaped dose-dependency and could revert JA-induced bundling. Impeded cell expansion as a consequence of stress treatment or superoptimal auxin was linked with the appearance of intranuclear tubulin speckles. The early and stimulus-specific responses of MTs are discussed with respect to a function in processing or decoding of stress signals.
0176-1617
Auxin
Green fluorescent protein (GFP)
Grapevine, transgenic
QK711.2 JOU
Tubulin marker line of grapevine suspension cells as a tool to follow early stress responses created by Xin Guan, Günther Buchholz and Peter Nick - Journal of plant physiology Volume 176 .
Plant microtubules (MTs), in addition to their role in cell division and cell expansion, respond to various stress signals. To understand the biological function of this early response requires non-destructive strategies for visualization in cellular models that are highly responsive to stress signals. We have therefore generated a transgenic tubulin marker line for a cell line from the grapevine Vitis rupestris that readily responds to stress factors of defense-related and abiotic stresses based on a fusion of the green fluorescent protein with Arabidopsis β-tubulin 6. By a combination of spinning-disk confocal microscopy with quantitative image analysis, we could detect early and specific responses of MTs to defense-related and abiotic stress factors in vivo. We observed that Harpin Z (HrpZ), a bacterial elicitor that can trigger programmed cell death, rapidly eliminated radial MTs, followed by a slower depletion of the cortical array. Jasmonic acid (JA), in contrast, induced bundling of cortical MTs. Auxin reduced the thickness of cortical MTs. This effect followed a characteristic bell-shaped dose-dependency and could revert JA-induced bundling. Impeded cell expansion as a consequence of stress treatment or superoptimal auxin was linked with the appearance of intranuclear tubulin speckles. The early and stimulus-specific responses of MTs are discussed with respect to a function in processing or decoding of stress signals.
0176-1617
Auxin
Green fluorescent protein (GFP)
Grapevine, transgenic
QK711.2 JOU